Nasal Spray Reconstitution Kit

What the kit is for

Why a dedicated diluent is used

Plain bacteriostatic water is hypotonic and unbuffered. That is acceptable for a small vial that is drawn from with a syringe, but a spray-format preparation is exposed to air with every actuation and often sits at room temperature during handling. The kit's diluent is isotonic, pH-buffered and preserved, which helps limit peptide degradation and microbial growth in the bottle. Researchers should still confirm compatibility with any specific peptide by checking its COA and their own stability data.

Preparing a spray-format solution

• Reconstitute the lyophilized peptide vial with a small volume of the diluent (or bacteriostatic water) so the cake dissolves completely
• Transfer the concentrate to the spray bottle and bring it up to the intended volume with additional diluent
• Fit the pump, prime it several times into a waste container, then label the bottle with peptide, concentration, date and lot
• Concentration per actuation is calculated from total peptide mass, final volume and the pump's metered volume

Peptides commonly prepared this way

Semax and Selank, the two heptapeptides developed in Russian research programs, are almost always studied in spray format, which is why the kit is bundled with those products. Oxytocin, PT-141 and NAD+ preparations also appear in the research literature in spray or intranasal-model form. The kit lets a laboratory prepare any of these from bulk lyophilized vials rather than buying pre-filled bottles.

Keep the sealed diluent in a cool, dry place away from light and do not use it if it appears cloudy. Work on a clean surface, wipe vial stoppers with an alcohol prep pad, and use a fresh sterile syringe for each transfer into the spray bottle. After assembling and priming the pump, replace the dust cap, apply a label with contents and date, and store the filled bottle upright at 2–8 °C. Do not top up a partially used bottle with fresh solution; prepare a new bottle instead. Discard bottles at the end of the study window and place any needles used during preparation in a sharps container.